Tapestation for Mcap larvae C/D

I ran the Tapestation today based on Maggie’s tapestation protocol. I ran 5 samples from AH’s experiment that failed the Qubit. The samples that I ran were R43, R54, R59, R88, and R94. R43 was extracted on 10/5/23, R59 & R94 were extracted on 9/11/23, and R54 & R88 were extracted on 9/16/23

Amy and I ran the Tapestation together so the first 4 and last 1 samples on the gel are my samples. All the samples have distinct bands, just like on the gels that I ran:

I got pretty solid RIN values for all the samples! And the concentrations look good too.

Here are the individual plots for the 4 samples:

R43

R54

R59

R88

R94

All of these samples failed the Qubit, but got really good concentrations on the Tapestation. I still need to run a sample that passed Qubit on the Tapestation to compare.

Written on October 5, 2023