January 2025 Goals

January 2025 goals and daily notebook entries.

Goals

Writing and manuscripts

  1. e5 ncRNA complete draft of manuscript submit to biorxiv
  2. Pacuta manuscript submit to biorixiv
  3. Write up Mcap 2023 results
  4. Resubmit Astrangia review by Jan 15
  5. CBASS results + discussion

Sample processing

  1. Process e5 timeseries samples for lipids + metabolites by Jan 15
  2. Mcap phys stuff ?

Data analysis

  1. Look into trimming for Mcap data
  2. Double check all Mcap data
  3. CBASS survey + ED50 data

Field work / travel

  1. SICB
  2. Fill out TERs (CBASS and sicb)

Daily log

20250101

  • Working on SICB presentation
  • Rerunning mirdeep2 mapper.pl script for Mcap data

20250102

  • Mcap data analysis - trimming to 35bp for raw data and running mirdeep2 on reads trimmed to 75bp
  • Travel to GA

20250103

  • Mcap data analysis (wgcna for mrna)
  • Working on sicb presentation
  • SICB

20250104 - 20250107

SICB!

20250108

  • Travel to RI
  • Submission of Pacuta paper to biorixiv
  • Prepping submission for molecular ecology

20250109

  • Edits to Astrangia review
  • Some stuff for Apul methylation
  • Emails

20250110

  • Apul methylation plotting

20250112

  • Apul methylation plotting and writing

20250113

  • Helping Zoe and Hollie prep for NYC
  • Astrangia review edits
  • Submitted Pacuta 2022 to molecular ecology
  • Pacuta paper out on biorvix!
  • CBASS edits + vba
  • Email catch up

20250114

  • Lauren meeting
  • Astrangia review edits
  • Therapy

20250115

  • Writing time
  • Lipid metabolite e5 sample prep (6 samples)

20250116

  • Writing with Hollie - Astrangia review

20250117

  • Cutting words for Astrangia review
  • e5 meeting
  • Lipid metabolite e5 sample prep (18 samples)

20250118

  • Resubmitted Pacuta paper (w/ line numbers)
  • Submitted Astrangia review revisions
  • Lipid metabolite e5 sample prep (12 samples)

20250119

  • Submitted epimar abstract
  • Lipid metabolite e5 sample prep (12 samples) - finished!

Notes:

GSA for maldives ask Hollie for cfs for maldives sicb put difference on intern uber:8.10+10.19+7.29+8.48+10.21+8.95

polyA for Mcap tapestation and qubit for every sample – 32 samples for me, #1-32, ask what concentrations to pool at – will be prepared to pool at specific molarity

  • https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-reference_material-list/000001240
Written on January 1, 2025