ITS2 sequencing plates
ITS2 samples + plate maps for sequencing
This post has information about the ITS2 samples that were sent for sequencing, along with the plate maps. The samples came from Ariana’s larvae C/D experiment, my Mcap DT 2023 experiment, POC spawning 2023, and Stefane’s stuff. Larvae C/D github repo is here; Mcap DT 2023 github repo is here; POC github is here. See previous posts (ITS2 amplification and clean up) for more information about the PCR and clean-up steps of the protocol.
Now that the samples have been properly amplified and cleaned, they are ready to be sent to Janet at RI INBRE Molecular Informatics Core for ITS2 amplicon sequencing.
Samples
- R## samples = Ariana samples
- M## = my samples
- Number## = POC samples
- S## = Stefane’s samples
| Plate | Well | Sample ID | Sequencing ID | Volume (uL) | Type |
|---|---|---|---|---|---|
| 1 | A1 | R55 | HP801 | 20 | ITS2 amplicon |
| 1 | B1 | R56 | HP802 | 20 | ITS2 amplicon |
| 1 | C1 | R57 | HP803 | 20 | ITS2 amplicon |
| 1 | D1 | R58 | HP804 | 20 | ITS2 amplicon |
| 1 | E1 | R59 | HP805 | 20 | ITS2 amplicon |
| 1 | F1 | R60 | HP806 | 20 | ITS2 amplicon |
| 1 | G1 | R61 | HP807 | 20 | ITS2 amplicon |
| 1 | H1 | R62 | HP808 | 20 | ITS2 amplicon |
| 1 | A2 | R63 | HP809 | 20 | ITS2 amplicon |
| 1 | B2 | R64 | HP810 | 20 | ITS2 amplicon |
| 1 | C2 | R65 | HP811 | 20 | ITS2 amplicon |
| 1 | D2 | R66 | HP812 | 20 | ITS2 amplicon |
| 1 | E2 | R67 | HP813 | 20 | ITS2 amplicon |
| 1 | F2 | R68 | HP814 | 20 | ITS2 amplicon |
| 1 | G2 | R69 | HP815 | 20 | ITS2 amplicon |
| 1 | H2 | R70 | HP816 | 20 | ITS2 amplicon |
| 1 | A3 | R71 | HP817 | 20 | ITS2 amplicon |
| 1 | B3 | R72 | HP818 | 20 | ITS2 amplicon |
| 1 | C3 | M48 | HP819 | 20 | ITS2 amplicon |
| 1 | D3 | M39 | HP820 | 20 | ITS2 amplicon |
| 1 | E3 | M73 | HP821 | 20 | ITS2 amplicon |
| 1 | F3 | M9 | HP822 | 20 | ITS2 amplicon |
| 1 | G3 | 107 | HP823 | 20 | ITS2 amplicon |
| 1 | H3 | 108 | HP824 | 20 | ITS2 amplicon |
| 1 | A4 | M51 | HP825 | 20 | ITS2 amplicon |
| 1 | B4 | M14 | HP826 | 20 | ITS2 amplicon |
| 1 | C4 | M60 | HP827 | 20 | ITS2 amplicon |
| 1 | D4 | 8 | HP828 | 20 | ITS2 amplicon |
| 1 | E4 | M23 | HP829 | 20 | ITS2 amplicon |
| 1 | F4 | M72 | HP830 | 20 | ITS2 amplicon |
| 1 | G4 | M52 | HP831 | 20 | ITS2 amplicon |
| 1 | H4 | M8 | HP832 | 20 | ITS2 amplicon |
| 1 | A5 | 110 | HP833 | 20 | ITS2 amplicon |
| 1 | B5 | M35 | HP834 | 20 | ITS2 amplicon |
| 1 | C5 | M63 | HP835 | 20 | ITS2 amplicon |
| 1 | D5 | M28 | HP836 | 20 | ITS2 amplicon |
| 1 | E5 | 106 | HP837 | 20 | ITS2 amplicon |
| 1 | F5 | 111 | HP838 | 20 | ITS2 amplicon |
| 1 | G5 | M6 | HP839 | 20 | ITS2 amplicon |
| 1 | H5 | M13 | HP840 | 20 | ITS2 amplicon |
| 1 | A6 | 112 | HP841 | 20 | ITS2 amplicon |
| 1 | B6 | M83 | HP842 | 20 | ITS2 amplicon |
| 1 | C6 | M24 | HP843 | 20 | ITS2 amplicon |
| 1 | D6 | M36 | HP844 | 20 | ITS2 amplicon |
| 1 | E6 | M61 | HP845 | 20 | ITS2 amplicon |
| 1 | F6 | M88 | HP846 | 20 | ITS2 amplicon |
| 1 | G6 | M74 | HP847 | 20 | ITS2 amplicon |
| 1 | H6 | M75 | HP848 | 20 | ITS2 amplicon |
| 1 | A7 | M47 | HP849 | 20 | ITS2 amplicon |
| 1 | B7 | M62 | HP850 | 20 | ITS2 amplicon |
| 1 | C7 | M86 | HP851 | 20 | ITS2 amplicon |
| 1 | D7 | M7 | HP852 | 20 | ITS2 amplicon |
| 1 | E7 | M37 | HP853 | 20 | ITS2 amplicon |
| 1 | F7 | M10 | HP854 | 20 | ITS2 amplicon |
| 1 | G7 | M26 | HP855 | 20 | ITS2 amplicon |
| 1 | H7 | 9 | HP856 | 20 | ITS2 amplicon |
| 1 | A8 | 6 | HP857 | 20 | ITS2 amplicon |
| 1 | B8 | M11 | HP858 | 20 | ITS2 amplicon |
| 1 | C8 | 113 | HP859 | 20 | ITS2 amplicon |
| 1 | D8 | 7 | HP860 | 20 | ITS2 amplicon |
| 1 | E8 | 109 | HP861 | 20 | ITS2 amplicon |
| 1 | F8 | M87 | HP862 | 20 | ITS2 amplicon |
| 1 | G8 | S1 | HP863 | 20 | ITS2 amplicon |
| 1 | H8 | S2 | HP864 | 20 | ITS2 amplicon |
| 1 | A9 | S3 | HP865 | 20 | ITS2 amplicon |
| 1 | B9 | S4 | HP866 | 20 | ITS2 amplicon |
| 1 | C9 | S5 | HP867 | 20 | ITS2 amplicon |
| 1 | D9 | S6 | HP868 | 20 | ITS2 amplicon |
| 1 | E9 | S7 | HP869 | 20 | ITS2 amplicon |
| 1 | F9 | S8 | HP870 | 20 | ITS2 amplicon |
| 1 | G9 | S9 | HP871 | 20 | ITS2 amplicon |
| 1 | H9 | S10 | HP872 | 20 | ITS2 amplicon |
| 1 | A10 | S11 | HP873 | 20 | ITS2 amplicon |
| 1 | B10 | S12 | HP874 | 20 | ITS2 amplicon |
| 1 | C10 | S13 | HP875 | 20 | ITS2 amplicon |
| 1 | D10 | S14 | HP876 | 20 | ITS2 amplicon |
| 1 | E10 | S15 | HP877 | 20 | ITS2 amplicon |
| 1 | F10 | S16 | HP878 | 20 | ITS2 amplicon |
| 1 | G10 | S17 | HP879 | 20 | ITS2 amplicon |
| 1 | H10 | S18 | HP880 | 20 | ITS2 amplicon |
| 1 | A11 | S19 | HP881 | 20 | ITS2 amplicon |
| 1 | B11 | S20 | HP882 | 20 | ITS2 amplicon |
| 1 | C11 | S21 | HP883 | 20 | ITS2 amplicon |
| 1 | D11 | S22 | HP884 | 20 | ITS2 amplicon |
| 1 | E11 | S23 | HP885 | 20 | ITS2 amplicon |
| 1 | F11 | S24 | HP886 | 20 | ITS2 amplicon |
| 1 | G11 | S25 | HP887 | 20 | ITS2 amplicon |
| 1 | H11 | S26 | HP888 | 20 | ITS2 amplicon |
| 1 | A12 | S27 | HP889 | 20 | ITS2 amplicon |
| 1 | B12 | S28 | HP890 | 20 | ITS2 amplicon |
| 1 | C12 | S29 | HP891 | 20 | ITS2 amplicon |
| 1 | D12 | S30 | HP892 | 20 | ITS2 amplicon |
| 1 | E12 | S31 | HP893 | 20 | ITS2 amplicon |
| 1 | F12 | S32 | HP894 | 20 | ITS2 amplicon |
| 1 | G12 | S33 | HP895 | 20 | ITS2 amplicon |
| 1 | H12 | S34 | HP896 | 20 | ITS2 amplicon |
| 2 | A1 | S35 | HP901 | 20 | ITS2 amplicon |
| 2 | B1 | S36 | HP902 | 20 | ITS2 amplicon |
| 2 | C1 | S37 | HP903 | 20 | ITS2 amplicon |
| 2 | D1 | S38 | HP904 | 20 | ITS2 amplicon |
| 2 | E1 | S39 | HP905 | 20 | ITS2 amplicon |
| 2 | F1 | S40 | HP906 | 20 | ITS2 amplicon |
Plate maps
Written on May 20, 2024