Goals: create spawning and fertilization protocol for Astrangia 2021

GSO Astrangia Experiment: Spawning & Fertilization

About: This post outlines the steps for spawning and fertilization in Astrangia poculata.

Materials & Equipment needed

• 50 and 15 ml falcon tubes
• Tube racks
• Cryotubes
• Cryotube boxes
• Squirt bottles
• 10, 20, 1000 ul pipettes and tips
• Transfer pipettes
• Loligo plates
• Glass bowls
• Dissecting microscope
• Banjo filters
• 53, 63, 153 um mesh and sieves
• 1/2’’ PVC pipe, couplers, and elbows
• Water line tubing
• Squaricals
• Turkey basters
• 4% paraformaldehyde
• 20% Z-fix
• 70% ethanol
• DI water

Before getting started…

• Think critically about your experiment and make a tiered plan for data collection. There is a lot that can go awry during spawning and it’s important to be able to make game-time decisions.
• Prep all materials! Label tubes, make datasheets, etc. See how to make squaricals here.

Protocol Steps